The purpose of this study was to evaluate whether removal of gingival epithelium with pulsed Nd: YAG laser could inhibit the downgrowth of junctional epithelium after alloplastic material grafting in periodontal bone defect.
The periodontal bone defects were created surgically on the palatal aspect of the upper right and left molar teeth ion 30 rats and filled with resorbable calcium carbonate (Biocoral 450(r): Inoteb, France). The control sites(right molar area) was
sutured. The test side (left molar area) received controlled de-epithelization of the and sulcular epithelium with pulsed Nd: YAG laser(Sunrise Master(r): Sunrise Technilogies. U.S.A) under the mode of 1.75W, 15Hz, 116mJ/pulse and was sutured.
The
control and test sites were evaluated clinically and histologically, at 1, 3, 7, 14, and 28 days postoperation.
Clinically, the gingiva showed normal color and shape at the 5th day in the control site and at the 10th day in the test sites.
Histologically, the junctional epithelium was formed at the 7th day in the control sites and at the 14th day in the test sites, and the long JE attachment were observed at the 28th day in both sites. The attachment of connective tissue to root
surface
was observed initially at the 7th day in the control sites and at the 14th day in the test sites, and completed at the 28th day in both sites. In summary, these results showed that the removal of oral epithelium using pulsed Nb: YAG Laser could
not
prevent epithelial downgrowth after alloplastic material implantation in rat periodontal bone defect.
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